Description
The rT3 ELISA is a competitive enzyme immunoassay, where the antigen (rT3 present in calibrators, controls and samples) competes with a biotin-labelled antigen (rT3-Biotin conjugate) for a limited quantity of antibody which is coated on the microplate wells. After one hour incubation followed by the first washing, unbound materials are removed and a Streptavidin-HRP conjugate is added and incubated for 30 minutes. Following a second washing, the TMB substrate is added. The enzymatic reaction is terminated by addition of the stopping solution, upon which the color intensity is measured with a microplate reader. The color intensity is inversely proportional to the concentration of rT3 in the sample. The set of kit calibrators that are run simultaneously with the samples is used to plot a calibration curve and determine the concentration of rT3 in samples and controls.
For the determination of Reverse Triiodothyronine (rT3) in human serum and plasma by an enzyme immunoassay